#!/usr/bin/env perl use strict; use File::Basename qw(basename); use File::Glob qw(:bsd_glob); use File::Path qw(make_path remove_tree); use Getopt::Long; binmode STDOUT; my ($in, $rna, $wrapper_only); GetOptions( 'in=s' => \$in, 'rna' => \$rna, 'wrapper-only' => \$wrapper_only, ) or die "Can't parse command line arguments\n"; if (!defined $in) { die "Input file is not specified\n"; } if (!-e $in or !-f $in) { die "Can't find the input file\n"; } my $name = basename($in); $name =~ s/\s/_/g; my $temp_dir = $ENV{'TMPDIR'}; if (!defined $temp_dir) { die "Environment variable 'TMPDIR' is not set\n"; } if (!-e $temp_dir or !-d $temp_dir) { die "Can't find temporary directory\n"; } my $new_temp_dir = "$temp_dir/$name-beetl-d-dir"; make_path($new_temp_dir); $ENV{'TMP'} = $new_temp_dir; $ENV{'TEMP'} = $new_temp_dir; $ENV{'TMPDIR'} = $new_temp_dir; my $len = `head -n 1 '$in.line-length'`; my $temp_prefix = "$new_temp_dir/$name"; my $temp_names = "$temp_prefix-names"; my $temp_lengths = "$temp_prefix-lengths"; my $temp_soft_mask = "$temp_prefix-soft-mask"; my $temp_n = "$temp_prefix-n"; my $temp_iupac = "$temp_prefix-iupac"; my $temp_seq_head = "$temp_prefix-seq-head"; my $temp_seq_tail = "$temp_prefix-seq-tail"; if ($wrapper_only) { run("sequence-merge-to-one-line <'$in' >'$temp_seq_head'"); } else { my @archives = bsd_glob("$in.beetl-B*"); if (scalar(@archives) > 0) { foreach my $archive (@archives) { my $file = $new_temp_dir . '/' . basename($archive); $file =~ s/\.[^.]+$//; run("zstd -dc <'$archive' >'$file'"); } my $beetl_name = "$new_temp_dir/$name.beetl"; my $temp_unbeetl = "$temp_prefix-unbeetl"; my $cmd = "beetl-unbwt -i '$beetl_name' -o '$temp_unbeetl' --output-format fasta --temp-directory '$new_temp_dir' --no-temp-subdir >/dev/null 2>&1"; run($cmd); if (!-e $temp_unbeetl) { die "Can't find \"$temp_unbeetl\" after \"$cmd\"\n"; } run("fasta-to-sequence <'$temp_unbeetl' >'$temp_seq_head'"); } else { system(":>'$temp_seq_head'"); } } run("zstd -dc <'$in.lengths' >'$temp_lengths'"); run("zstd -dc <'$in.names' >'$temp_names'"); run("zstd -dc <'$in.soft-mask' >'$temp_soft_mask'"); run("zstd -dc <'$in.n' >'$temp_n'"); run("zstd -dc <'$in.iupac' >'$temp_iupac'"); run("zstd -dc <'$in.tail' >'$temp_seq_tail'"); my $cmd = "cat '$temp_seq_head' '$temp_seq_tail'" . ($rna ? ' | sequence-t2u' : '') . " | sequence-iupac-add --iupac '$temp_iupac'" . " | sequence-n-add --n '$temp_n'" . " | sequence-soft-mask-add --mask '$temp_soft_mask'" . " | fasta-from-names-lengths-sequence --names '$temp_names' --lengths '$temp_lengths' --line-length $len"; run($cmd); remove_tree($new_temp_dir); sub run { my ($cmd) = @_; my $error = system($cmd); if ($error) { die "Command failed:\n$cmd\n"; } }