#!/usr/bin/env perl use strict; use Cwd qw(cwd); use File::Basename qw(basename); use File::Copy qw(move); use File::Path qw(make_path remove_tree); use Getopt::Long; my ($out, $level, $wrapper_only); GetOptions( 'out=s' => \$out, 'level=i' => \$level, 'wrapper-only' => \$wrapper_only, ) or die "Can't parse command line arguments\n"; if (!defined $out) { die "Output file is not specified\n"; } if (!defined $level) { die "Level is not specified\n"; } my $name = basename($out); $name =~ s/\s/_/g; my $temp_dir = $ENV{'TMPDIR'}; if (!defined $temp_dir) { die "Environment variable 'TMPDIR' is not set\n"; } if (!-e $temp_dir or !-d $temp_dir) { die "Can't find temporary directory\n"; } my $start_dir = cwd(); my $new_temp_dir = "$temp_dir/$name-dir"; make_path($new_temp_dir); $ENV{'TMP'} = $new_temp_dir; $ENV{'TEMP'} = $new_temp_dir; $ENV{'TMPDIR'} = $new_temp_dir; my $temp_prefix = "$new_temp_dir/$name"; my $temp_fasta = "$temp_prefix.fa"; my $temp_names = "$temp_prefix-names"; my $temp_lengths = "$temp_prefix-lengths"; my $temp_soft_mask = "$temp_prefix-soft-mask"; my $temp_n = "$temp_prefix-n"; my $temp_iupac = "$temp_prefix-iupac"; my $temp_sequence = "$temp_prefix"; my $buf_size = 1000000; my $buffer; binmode STDIN; open(my $FA, '>', $temp_fasta) or die "Can't create temporary file \"$temp_fasta\"\n"; binmode $FA; while (read(STDIN, $buffer, $buf_size)) { print $FA $buffer; } close $FA; run("fasta-guess-line-length.pl '$temp_fasta' >'$out.line-length'"); my $cmd = "fasta-to-names-lengths-sequence --names '$temp_names' --lengths '$temp_lengths' <'$temp_fasta'" . " | sequence-soft-mask-remove --mask '$temp_soft_mask'" . " | sequence-n-remove --n '$temp_n'" . " | sequence-iupac-remove --iupac '$temp_iupac'" . " >'$temp_sequence'"; run($cmd); run("zstd -c -1 <'$temp_names' >'$out.names'"); run("zstd -c -1 <'$temp_lengths' >'$out.lengths'"); run("zstd -c -1 <'$temp_soft_mask' >'$out.soft-mask'"); run("zstd -c -1 <'$temp_n' >'$out.n'"); run("zstd -c -1 <'$temp_iupac' >'$out.iupac'"); if (!chdir($new_temp_dir)) { die "Can't change directory to \"$new_temp_dir\"\n"; } if (!$wrapper_only) { run("ulimit -s unlimited; dna-compact n -$level '$name' >/dev/null 2>&1"); } if (!chdir($start_dir)) { die "Can't change directory to \"$start_dir\"\n"; } if ($wrapper_only) { move($temp_sequence, $out); } else { my $temp_dcom_1 = "$temp_sequence.fp"; if (!-e $temp_dcom_1) { die "Can't find \"$temp_dcom_1\"\n"; } my $temp_dcom_2 = "$temp_sequence.fp.txt.second"; if (!-e $temp_dcom_2) { die "Can't find \"$temp_dcom_2\"\n"; } move($temp_dcom_1, "$out.fp"); move($temp_dcom_2, $out); unlink $temp_sequence; } unlink $temp_fasta; unlink $temp_names; unlink $temp_lengths; unlink $temp_soft_mask; unlink $temp_n; unlink $temp_iupac; remove_tree($new_temp_dir); sub run { my ($cmd) = @_; my $error = system($cmd); if ($error) { die "Command failed:\n$cmd\n"; } }