#!/usr/bin/env perl use strict; use File::Basename qw(basename); use File::Copy qw(move); use Getopt::Long; my ($out, $read_length, $threads, $rna, $wrapper_only); GetOptions( 'out=s' => \$out, 'read-length=i' => \$read_length, 'threads=i' => \$threads, 'rna' => \$rna, 'wrapper-only' => \$wrapper_only, ) or die "Can't parse command line arguments\n"; if (!defined $out) { die "Output file is not specified\n"; } if (!defined $read_length) { die "Read length is not specified\n"; } if ($read_length < 1 or $read_length > 100000) { die "Read length is out of range\n"; } if (!defined $threads) { die "Number of threads is not specified\n"; } my $name = basename($out); $name =~ s/\s/_/g; my $temp_dir = $ENV{'TMPDIR'}; if (!defined $temp_dir) { die "Environment variable 'TMPDIR' is not set\n"; } if (!-e $temp_dir or !-d $temp_dir) { die "Can't find temporary directory\n"; } my $temp_prefix = "$temp_dir/$name"; my $temp_fasta = "$temp_prefix-fa"; my $temp_names = "$temp_prefix-names"; my $temp_lengths = "$temp_prefix-lengths"; my $temp_soft_mask = "$temp_prefix-soft-mask"; my $temp_n = "$temp_prefix-n"; my $temp_iupac = "$temp_prefix-iupac"; my $temp_fastq = "$temp_prefix-fastq"; my $buf_size = 1000000; my $buffer; binmode STDIN; open(my $FA, '>', $temp_fasta) or die "Can't create temporary file \"$temp_fasta\"\n"; binmode $FA; while (read(STDIN, $buffer, $buf_size)) { print $FA $buffer; } close $FA; run("fasta-guess-line-length.pl '$temp_fasta' >'$out.line-length'"); my $cmd = "fasta-to-names-lengths-sequence --names '$temp_names' --lengths '$temp_lengths' <'$temp_fasta'" . " | sequence-soft-mask-remove --mask '$temp_soft_mask'" . " | sequence-n-remove --n '$temp_n'" . " | sequence-iupac-remove --iupac '$temp_iupac'" . ($rna ? ' | sequence-u2t' : '') . " | fastq-from-sequence --seq-length $read_length --quality A >'$temp_fastq'"; run($cmd); run("zstd -c -1 <'$temp_names' >'$out.names'"); run("zstd -c -1 <'$temp_lengths' >'$out.lengths'"); run("zstd -c -1 <'$temp_soft_mask' >'$out.soft-mask'"); run("zstd -c -1 <'$temp_n' >'$out.n'"); run("zstd -c -1 <'$temp_iupac' >'$out.iupac'"); if ($wrapper_only) { move($temp_fastq, $out); } else { my $cmd = "fqs-0.1 e -s -t $threads -out $out -om o -qm n -im n -tmp $temp_dir '$temp_fastq' >/dev/null 2>&1"; run($cmd); unlink $temp_fastq; } unlink $temp_fasta; unlink $temp_names; unlink $temp_lengths; unlink $temp_soft_mask; unlink $temp_n; unlink $temp_iupac; sub run { my ($cmd) = @_; my $error = system($cmd); if ($error) { die "Command failed:\n$cmd\n"; } }