#!/usr/bin/env perl use strict; use File::Basename qw(basename); use Getopt::Long; binmode STDOUT; my ($in, $rna, $wrapper_only); GetOptions( 'in=s' => \$in, 'rna' => \$rna, 'wrapper-only' => \$wrapper_only, ) or die "Can't parse command line arguments\n"; if (!defined $in) { die "Input file is not specified\n"; } if (!-e $in or !-f $in) { die "Can't find the input file\n"; } my $name = basename($in); $name =~ s/\s/_/g; my $temp_dir = $ENV{'TMPDIR'}; if (!defined $temp_dir) { die "Environment variable 'TMPDIR' is not set\n"; } if (!-e $temp_dir or !-d $temp_dir) { die "Can't find temporary directory\n"; } my $len = `head -n 1 '$in.line-length'`; my $temp_prefix = "$temp_dir/$name.alapy-d"; my $temp_names = "$temp_prefix-names"; my $temp_lengths = "$temp_prefix-lengths"; my $temp_soft_mask = "$temp_prefix-soft-mask"; my $temp_iupac = "$temp_prefix-iupac"; run("zstd -dc <'$in.lengths' >'$temp_lengths'"); run("zstd -dc <'$in.names' >'$temp_names'"); run("zstd -dc <'$in.soft-mask' >'$temp_soft_mask'"); run("zstd -dc <'$in.iupac' >'$temp_iupac'"); my $temp_fastq; if ($wrapper_only) { $temp_fastq = $in; } else { my $cmd = "java -jar /data/kirill/tools/kic/kid.jar '$in' >/dev/null 2>&1"; run($cmd); $temp_fastq = "$in.fq"; if (!-e $temp_fastq) { die "Can't find decompressed fastq file after command:\n$cmd\n"; } } my $cmd = "fastq-to-sequence <'$temp_fastq'" . ($rna ? ' | sequence-t2u' : '') . " | sequence-iupac-add --iupac '$temp_iupac'" . " | sequence-soft-mask-add --mask '$temp_soft_mask'" . " | fasta-from-names-lengths-sequence --names '$temp_names' --lengths '$temp_lengths' --line-length $len"; run($cmd); if (!$wrapper_only) { unlink $temp_fastq; } unlink $temp_names; unlink $temp_lengths; unlink $temp_soft_mask; unlink $temp_iupac; sub run { my ($cmd) = @_; my $error = system($cmd); if ($error) { die "Command failed:\n$cmd\n"; } }